death, however, any sample is better than none and the lab does not charge based on the number of
samples submitted. Both fresh and formalin fixed tissues from any gross lesions and the following tissues
are recommended for submission
The ideal samples for investigation of enteric diseases are those that are collected within 4-8 hours of
death, however, any sample is better than none and the lab does not charge based on the number of
samples submitted. Both fresh and formalin fixed tissues from any gross lesions and the following tissues
are recommended for submission
Pathology Price List
File: CL-Res-21-Pathology-Price-Sheet.pdfThe ideal samples for investigation of respiratory diseases are those that are collected within 4-8
hours of death, however, any sample is better than none, and the lab does not charge based on
the number of samples submitted. Both fresh and formalin fixed tissues from 2-4 different sections
of lung including the junction between normal and diseased tissue.
The ideal samples are those that are collected within 8-12 hours of fetal expulsion although
samples collected 1-2 days after abortion may still yield useful information. Submit either the
entire fetus and placenta or both fresh and formalin fixed tissues as outlined.
Neospora caninum is a major cause of abortions in cattle. First recognized in 1988, and linked to dogs in
1998, this parasite causes an infection called neosporosis. Studies have shown that at least half the dairy
and beef herds in the United States have one or more animals that have been exposed. In an infected
herd, up to 30 percent of the animals may test positive, and some cows may abort several times. With
good herd management, though, you can reduce this drain on your profits.
In a real time PCR assay a positive reaction is detected by accumulation of a fluorescent signal. The CT (cycle
threshold) is defined as the number of cycles required for the fluorescent signal to exceed background levels and
cross a pre-set threshold. CT levels are inversely proportional to the amount of target nucleic acid detected in the
sample (i.e., the lower the CT level the greater the amount of target nucleic acid in the sample).
WVDL real time assays undergo 40 cycles of amplification.
Pharyngeal Swab
File: FM-CL-ORD-1-PHARYNGEALSWABORDER-1.pdfThe Wisconsin Veterinary Diagnostic Laboratory (WVDL) is committed to providing the highest quality veterinary diagnostic services which meet or exceed client and regulatory requirements.
File: FM-Q-12-Quality-Policy-Statement.pdfThere are many methodologies in determining a current infection with Salmonella enterica serotype Dublin. These include conventional culture with serotyping or polymerase chain reaction (PCR). In addition, diagnostic laboratories in the United States and several countries in Europe also utilize an enzyme linked immunosorbent assay (ELISA) to measure the level of antibodies directed against O-antigens from Salmonella enterica serotype Dublin in both blood and milk samples. These ELISAs measure the humoral immune response as an indicator of current or previous infections. ELISA results are reported as a semi-quantitative percentage value, giving an optical density reading referable to a standard set of controls. Individual or bulk tank milk samples can also be conducted on ELISAs and have been used for screening / active surveillance programs for this serovar. Sensitivity for the serum ELISA is considerably higher than fecal culture for the identification of Salmonella enterica serotype Dublin infected cattle. As a diagnostic test, the serum ELISA is reported to perform best when used in animals between 3 and 10 months of age
File: CL-Res-89-Salmonella-Dublin-ELISA-Testing-At-WVDL.pdf