Background: Federal regulations and vendor policies exist prohibiting the use of barbiturates in euthanasia of animals disposed of by rendering or landfill.
Species affected and disposal options: The following species can be rendered, if not euthanized with barbiturates or diagnosed with a select agent: bovines <30 months of age (including fetuses), bovines > 30 months of age with brain and spinal cord removed, bovines > 30 months of age with a negative BSE test, small ruminants with a negative scrapie test, poultry, pigs, camelids, lagomorphs, and fish. In addition to animals euthanized with barbiturates or animals diagnosed with a select agent, the following species cannot be disposed of by rendering: cervids, horses, large wildlife and zoo animals. Incineration is a disposal option for domestic dogs and cats, mustelids, pocket pets, and small wildlife and zoo animals.
WVDL Zero Tolerance Barbiturate Policy
File: CL-Res-105-Necropsy-Barbiturate-Residue-Rendering-Guidelines.pdf
The best samples are collected within 4-8 hours of death, but it’s difficult to predict which cases will
be diagnostic. Please collect both fresh and formalin-fixed tissues from any organ that has gross
lesions.
File: CL-Res-35-Necropsy-Bottle-Necropsy-Sampling-Guidelines.pdf
death, however, any sample is better than none and the lab does not charge based on the number of
samples submitted. Both fresh and formalin fixed tissues from any gross lesions and the following tissues
are recommended for submission
File: CL-Res-29-Necropsy-Enteric-Disease-Sampling-Guidelines.pdf
The ideal samples for investigation of enteric diseases are those that are collected within 4-8 hours of
death, however, any sample is better than none and the lab does not charge based on the number of
samples submitted. Both fresh and formalin fixed tissues from any gross lesions and the following tissues
are recommended for submission
File: CL-Res-28-Necropsy-Neonatal-Calves-Sampling-Guidelines.pdf
Pathology Price List
File: CL-Res-21-Pathology-Price-Sheet.pdf
The ideal samples for investigation of respiratory diseases are those that are collected within 4-8
hours of death, however, any sample is better than none, and the lab does not charge based on
the number of samples submitted. Both fresh and formalin fixed tissues from 2-4 different sections
of lung including the junction between normal and diseased tissue.
File: CL-Res-37-Necropsy-Respiratory-Disease-Sampling-Guidelines.pdf
The ideal samples are those that are collected within 8-12 hours of fetal expulsion although
samples collected 1-2 days after abortion may still yield useful information. Submit either the
entire fetus and placenta or both fresh and formalin fixed tissues as outlined.
File: CL-Res-30-Necropsy-Ruminant-Abortion-Sampling-Guidelines.pdf
Neospora caninum is a major cause of abortions in cattle. First recognized in 1988, and linked to dogs in
1998, this parasite causes an infection called neosporosis. Studies have shown that at least half the dairy
and beef herds in the United States have one or more animals that have been exposed. In an infected
herd, up to 30 percent of the animals may test positive, and some cows may abort several times. With
good herd management, though, you can reduce this drain on your profits.
File: CL-Res-14-Recognizing-and-Preventing-Neosporosis-Infections.pdf
In a real time PCR assay a positive reaction is detected by accumulation of a fluorescent signal. The CT (cycle
threshold) is defined as the number of cycles required for the fluorescent signal to exceed background levels and
cross a pre-set threshold. CT levels are inversely proportional to the amount of target nucleic acid detected in the
sample (i.e., the lower the CT level the greater the amount of target nucleic acid in the sample).
WVDL real time assays undergo 40 cycles of amplification.
File: CL-Res-18-Real-Time-PCR-Ct-Values-1.pdf
Pharyngeal Swab
File: FM-CL-ORD-1-PHARYNGEALSWABORDER-1.pdf