PCR Test Interpretation Changes

With the implementation of the new LIMS system (COFL) at WVDL, molecular results will be reported differently than our previous LIMS system. Currently we report results as either positive, weak positive,  negative, inconclusive or undetermined. We will be changing that nomenclature to detected, not detected, inconclusive and indeterminate, which is in line with the reporting nomenclature that is use by NVSL (reference labs) and other veterinary diagnostic labs. A chart is included below as a reference. A guideline of PCR interpretations is also available on our website, www.wvdl.wisc.edu.

 

Previous LIMS PCR Interpretation New LIMS PCR Interpretation Ct Range
Positive Detected ≤ 37.0
Weak Positive or
Inconclusive
Inconclusive 37.1- 40.0
Undetermined
(MAP PCR only)
Indeterminate
(MAP PCR only)
No Ct
Negative Not Detected 0.0