Diagnostic Submission Guidelines: Enteric Disease
Wisconsin Veterinary Diagnostic Laboratory
by Dr. Donald Sockett
The best samples are those that are collected within 4-8 hours of the animal’s death. Delays in sample collection decrease the probability of obtaining useful diagnostic information. Veterinarians should submit both fresh and formalin fixed tissues from any organ that has gross lesions. See chart below for information on tissue types and how tissues should be collected and submitted to WVDL.
*Since the formalin jars tend to leak; please place the formalin jar inside a Ziploc® bag. If it is not possible to place the tissues in formalin at the time of sample collection, WVDL staff will do it for you. Please keep in mind the tissue samples will be more autolyzed and less diagnostic for histopathology when formalin fixation is delayed. A 2 x 2 x 1 cm piece of tissue from all the organs listed below should be placed in formalin. The ratio should be 9 parts formalin to 1 part tissue for proper fixation. The WVDL recommends using a 16-ounce wide-mouthed jar that contains 8 ounces of 10% buffered formalin (Fischer catalog # 23-426-797).
- Veterinarians should completely fill out the WVDL General Submission Form which includes the age of the animal. An electronic copy is available at www.wvdl.wisc.edu. Click on the forms link to download the submission form.
- The laboratory should receive the samples within 24-36 hours. See our website for discounted, expedited shipping options.
- Package the samples with a sufficient number of ice packs to ensure they remain cold during shipment.
- Clients should schedule shipments to avoid weekend and holiday delivery of samples to the laboratory.